Protein Counting Technique Improves Analysis Of Microscopy Images

Protein Counting with Super-Resolution Microscopy Techniques

Counting Proteins with Super-Resolution Microscopy

Super-resolution microscopy techniques offer unprecedented resolution, enabling researchers to visualize and count individual proteins within cells. However, analyzing high-density images of fluorescent proteins (FPs) can be challenging due to potential errors and complexities.

Simplifying Protein Data Analysis

To address these challenges, researchers have developed methods to simplify the analysis of protein data from microscopy images. These methods aim to minimize errors and provide accurate protein counts.

Quantitative Fluorescence Microscopy Methods

Three primary methods are used for determining absolute protein numbers using quantitative fluorescence microscopy:

• Direct stochastic optical reconstruction microscopy (dSTORM)
• Photoactivated localization microscopy (PALM)
• Bayesian analysis of blinking and bleaching (3B)

DOL Determination Technique

A novel technique called protein 1 Acquired images having both reference (DOL) determination has been developed to provide accurate protein counts. This technique utilizes images with both reference and target FPs to determine the absolute number of proteins in a sample.